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Fragmentation of genomic DNA (A) Agarose gel electrophoresis of genomic DNA before (lane 1) and after (lane 2-7) DNase I digestion with different incubation time. Successful digestion results in a smear of fragments ranging from 50 to 200 bp. M represents the DNA ladder, with numbers on the side indicating base pair in bp. (B) Electropherogram from the Agilent Bioanalyzer showing the size distribution of the DNase I-treated genomic DNA. After digestion for 3 min, 64.3% of the fragments are 50∼200 bp, and average size is 130 bp. (C) The virtual gel image corresponding to the electropherogram in (B). M represents the DNA ladder, with numbers on the left indicating base pair in bp.
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Fragmentation of genomic DNA (A) Agarose gel electrophoresis of genomic DNA before (lane 1) and after (lane 2-7) DNase I digestion with different incubation time. Successful digestion results in a smear of fragments ranging from 50 to 200 bp. M represents the DNA ladder, with numbers on the side indicating base pair in bp. (B) Electropherogram from the Agilent Bioanalyzer showing the size distribution of the DNase I-treated genomic DNA. After digestion for 3 min, 64.3% of the fragments are 50∼200 bp, and average size is 130 bp. (C) The virtual gel image corresponding to the electropherogram in (B). M represents the DNA ladder, with numbers on the left indicating base pair in bp.
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Fragmentation of genomic DNA (A) Agarose gel electrophoresis of genomic DNA before (lane 1) and after (lane 2-7) DNase I digestion with different incubation time. Successful digestion results in a smear of fragments ranging from 50 to 200 bp. M represents the DNA ladder, with numbers on the side indicating base pair in bp. (B) Electropherogram from the Agilent Bioanalyzer showing the size distribution of the DNase I-treated genomic DNA. After digestion for 3 min, 64.3% of the fragments are 50∼200 bp, and average size is 130 bp. (C) The virtual gel image corresponding to the electropherogram in (B). M represents the DNA ladder, with numbers on the left indicating base pair in bp.
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Fragmentation of genomic DNA (A) Agarose gel electrophoresis of genomic DNA before (lane 1) and after (lane 2-7) DNase I digestion with different incubation time. Successful digestion results in a smear of fragments ranging from 50 to 200 bp. M represents the DNA ladder, with numbers on the side indicating base pair in bp. (B) Electropherogram from the Agilent Bioanalyzer showing the size distribution of the DNase I-treated genomic DNA. After digestion for 3 min, 64.3% of the fragments are 50∼200 bp, and average size is 130 bp. (C) The virtual gel image corresponding to the electropherogram in (B). M represents the DNA ladder, with numbers on the left indicating base pair in bp.
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Fragmentation of genomic DNA (A) Agarose gel electrophoresis of genomic DNA before (lane 1) and after (lane 2-7) DNase I digestion with different incubation time. Successful digestion results in a smear of fragments ranging from 50 to 200 bp. M represents the DNA ladder, with numbers on the side indicating base pair in bp. (B) Electropherogram from the Agilent Bioanalyzer showing the size distribution of the DNase I-treated genomic DNA. After digestion for 3 min, 64.3% of the fragments are 50∼200 bp, and average size is 130 bp. (C) The virtual gel image corresponding to the electropherogram in (B). M represents the DNA ladder, with numbers on the left indicating base pair in bp.
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Fragmentation of genomic DNA (A) Agarose gel electrophoresis of genomic DNA before (lane 1) and after (lane 2-7) DNase I digestion with different incubation time. Successful digestion results in a smear of fragments ranging from 50 to 200 bp. M represents the DNA ladder, with numbers on the side indicating base pair in bp. (B) Electropherogram from the Agilent Bioanalyzer showing the size distribution of the DNase I-treated genomic DNA. After digestion for 3 min, 64.3% of the fragments are 50∼200 bp, and average size is 130 bp. (C) The virtual gel image corresponding to the electropherogram in (B). M represents the DNA ladder, with numbers on the left indicating base pair in bp.

Journal: STAR Protocols

Article Title: Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing

doi: 10.1016/j.xpro.2026.104513

Figure Lengend Snippet: Fragmentation of genomic DNA (A) Agarose gel electrophoresis of genomic DNA before (lane 1) and after (lane 2-7) DNase I digestion with different incubation time. Successful digestion results in a smear of fragments ranging from 50 to 200 bp. M represents the DNA ladder, with numbers on the side indicating base pair in bp. (B) Electropherogram from the Agilent Bioanalyzer showing the size distribution of the DNase I-treated genomic DNA. After digestion for 3 min, 64.3% of the fragments are 50∼200 bp, and average size is 130 bp. (C) The virtual gel image corresponding to the electropherogram in (B). M represents the DNA ladder, with numbers on the left indicating base pair in bp.

Article Snippet: 1Kb DNA Ladder , Thermo Fisher Scientific , Cat#10787026.

Techniques: Agarose Gel Electrophoresis, Incubation

Schematic representation of combined gene therapy for FD. α-Gal A: α-Galactosidase A. pDNA: Plasmid DNA. Gb3S: Globotriaosylceramide synthase. siRNA: Small interference RNA. FD: Fabry disease. gSRT: Genetic substrate reduction therapy. Gb3: Globotriaosylceramide.

Journal: International Journal of Pharmaceutics: X

Article Title: Nanomedicines for DNA and interference RNA co-delivery: Combined gene therapy for Fabry disease

doi: 10.1016/j.ijpx.2026.100555

Figure Lengend Snippet: Schematic representation of combined gene therapy for FD. α-Gal A: α-Galactosidase A. pDNA: Plasmid DNA. Gb3S: Globotriaosylceramide synthase. siRNA: Small interference RNA. FD: Fabry disease. gSRT: Genetic substrate reduction therapy. Gb3: Globotriaosylceramide.

Article Snippet: Agarose, deoxyribonuclease I (DNAse I) and sodium dodecyl sulfate (SDS) were purchased from Sigma-Aldrich, GelRedTM from Biotium (Fremont, CA, USA) and GeneRuler Ultra Low Range DNA Ladder and 1 Kb pDNA ladder from Gibco (Thermo Fisher Scientific, MA, USA).

Techniques: Plasmid Preparation

Binding, protection and release capacity of co-delivery vectors with SLNs containing DOTAP or DOTAP/MC3. In all gels, lane 1 corresponds to 1 Kb pDNA ladder. Lane 2 to naked pCMV6-AC-αGLA, lane 3 to naked pCMV6-AC-αGLA + SDS, lane 4 to naked siRNA, lane 5 to siRNA + SDS, lane 6 to siRNA-GN + SDS, lane 11 to naked pR-M10-αGal A and lane 12 to Naked siRNA + DNase + SDS. ( A ) DxP3GNSLNEE co-delivery vectors: (7) DxP3GN5-ET. (8) DxP3GN2.5-ET. (9) DxP3GN5-EMC3. (10) DxP3GN2.5-EMC3. (13) DxP3GN5-ET vector + DNase + SDS. (14) DxP3GN2.5-ET vector + DNase + SDS. (15) DxP3GN5-EMC3 + DNase + SDS. (16) DxP3GN2.5-EMC3 + DNase + SDS. (17) DxP3GN5-ET + SDS. (18) DxP3GN2.5-ET + SDS. (19) DxP3GN5-EMC3 + SDS. (20) DxP3GN2.5-EMC3 + SDS. (B) HAP3GNSLNEE co-delivery vectors: (7) HAP3GN5-ET. (8). HAP3GN2.5-ET. (9) HAP3GN5-EMC3. (10) HAP3GN2.5-EMC3. (13) HAP3GN5-ET + DNase + SDS. (14) HAP3GN2.5-ET + DNase + SDS. (15) HAP3GN5-EMC3 + DNase + SDS. (16) HAP3GN2.5-EMC3 + DNase + SDS. (17) HAP3GN5-ET + SDS. (18) HAP3GN2.5-ET + SDS. (19) HAP3GN5-EMC3 + SDS. (20) HAP3GN2.5-EMC3 + SDS. ( C ) DxP2GNSLNEE co-delivery vectors: (7) DxP2GN5-ET. (8). DxP2GN2.5-ET. (9) DxP2GN5-EMC3. (10) DxP2GN2.5-EMC3. (13) DxP3GN5-ET + DNase + SDS. (14) DxP3GN2.5-ET + DNase + SDS. (15) DxP2GN5-EMC3 + DNase + SDS. (16) DxP2GN2.5-EMC3 + DNase + SDS. (17) DxP2GN5-ET + SDS. (18) DxP2GN2.5-ET + SDS. (19) DxP2GN5-EMC3 + SDS. (20) DxP2GN2.5-EMC3 + SDS. ( D ) HAP2GNSLEE co-delivery vectors: (7) HAP2GN5-ET. (8). HAP2GN2.5-ET. (9) HAP2GN5-EMC3. (10) HAP2GN2.5-EMC3. (13) HAP2GN5-ET + DNase + SDS. (14) HAP2GN2.5-ET + DNase + SDS. (15) HAP2GN5-EMC3 + DNase + SDS. (16) HAP2GN2.5-EMC3 + DNase + SDS. (17) HAP2GN5-ET + SDS. (18) HAP2GN2.5-ET + SDS. (19) HAP2GN5-EMC3 + SDS. (20) HAP2GN2.5-EMC3 + SDS.

Journal: International Journal of Pharmaceutics: X

Article Title: Nanomedicines for DNA and interference RNA co-delivery: Combined gene therapy for Fabry disease

doi: 10.1016/j.ijpx.2026.100555

Figure Lengend Snippet: Binding, protection and release capacity of co-delivery vectors with SLNs containing DOTAP or DOTAP/MC3. In all gels, lane 1 corresponds to 1 Kb pDNA ladder. Lane 2 to naked pCMV6-AC-αGLA, lane 3 to naked pCMV6-AC-αGLA + SDS, lane 4 to naked siRNA, lane 5 to siRNA + SDS, lane 6 to siRNA-GN + SDS, lane 11 to naked pR-M10-αGal A and lane 12 to Naked siRNA + DNase + SDS. ( A ) DxP3GNSLNEE co-delivery vectors: (7) DxP3GN5-ET. (8) DxP3GN2.5-ET. (9) DxP3GN5-EMC3. (10) DxP3GN2.5-EMC3. (13) DxP3GN5-ET vector + DNase + SDS. (14) DxP3GN2.5-ET vector + DNase + SDS. (15) DxP3GN5-EMC3 + DNase + SDS. (16) DxP3GN2.5-EMC3 + DNase + SDS. (17) DxP3GN5-ET + SDS. (18) DxP3GN2.5-ET + SDS. (19) DxP3GN5-EMC3 + SDS. (20) DxP3GN2.5-EMC3 + SDS. (B) HAP3GNSLNEE co-delivery vectors: (7) HAP3GN5-ET. (8). HAP3GN2.5-ET. (9) HAP3GN5-EMC3. (10) HAP3GN2.5-EMC3. (13) HAP3GN5-ET + DNase + SDS. (14) HAP3GN2.5-ET + DNase + SDS. (15) HAP3GN5-EMC3 + DNase + SDS. (16) HAP3GN2.5-EMC3 + DNase + SDS. (17) HAP3GN5-ET + SDS. (18) HAP3GN2.5-ET + SDS. (19) HAP3GN5-EMC3 + SDS. (20) HAP3GN2.5-EMC3 + SDS. ( C ) DxP2GNSLNEE co-delivery vectors: (7) DxP2GN5-ET. (8). DxP2GN2.5-ET. (9) DxP2GN5-EMC3. (10) DxP2GN2.5-EMC3. (13) DxP3GN5-ET + DNase + SDS. (14) DxP3GN2.5-ET + DNase + SDS. (15) DxP2GN5-EMC3 + DNase + SDS. (16) DxP2GN2.5-EMC3 + DNase + SDS. (17) DxP2GN5-ET + SDS. (18) DxP2GN2.5-ET + SDS. (19) DxP2GN5-EMC3 + SDS. (20) DxP2GN2.5-EMC3 + SDS. ( D ) HAP2GNSLEE co-delivery vectors: (7) HAP2GN5-ET. (8). HAP2GN2.5-ET. (9) HAP2GN5-EMC3. (10) HAP2GN2.5-EMC3. (13) HAP2GN5-ET + DNase + SDS. (14) HAP2GN2.5-ET + DNase + SDS. (15) HAP2GN5-EMC3 + DNase + SDS. (16) HAP2GN2.5-EMC3 + DNase + SDS. (17) HAP2GN5-ET + SDS. (18) HAP2GN2.5-ET + SDS. (19) HAP2GN5-EMC3 + SDS. (20) HAP2GN2.5-EMC3 + SDS.

Article Snippet: Agarose, deoxyribonuclease I (DNAse I) and sodium dodecyl sulfate (SDS) were purchased from Sigma-Aldrich, GelRedTM from Biotium (Fremont, CA, USA) and GeneRuler Ultra Low Range DNA Ladder and 1 Kb pDNA ladder from Gibco (Thermo Fisher Scientific, MA, USA).

Techniques: Binding Assay, Plasmid Preparation